محلول Phosphate-buffered saline (PBS) 10X

صد میلی لیتر محلول غلیظ (10x) از PBS یا Phosphate-buffered saline برای ساخت یک لیتر PBS 1x

این محلول برای شستشوی سلول‌ها و به عنوان رقیق کننده در آزمایشات بیولوژی سلولی غیر استریل از جمله ایمونوسایتوکمیستری و الایزا کاربرد دارد.

pH این محلول ۷.۴ است.

100 ml of 10x PBS, pH 7.4 for ICC, IHC, and ELISA applications, enough to make 1 liter of 1x PBS | Cat. No. S-8012

DZ PBS (10x) is a concentrated PBS buffer that is made for diverse cell and molecular biology applications. It is provided as a 10x solution. To make a 1x solution, add the contents (100 ml) to a 1-liter container containing 900 ml of double-distilled water. The resulting 1x PBS buffer will be suitable for immunocytochemical, immunohistochemical, and molecular biology experiments. The pH of the concentrated (10x) solution is 6.8. After dilution to 1x PBS, the pH will change to 7.4. However, depending on the pH of water that is used for dilution, the final pH may be different. Thus, it is recommended to adjust the pH of the diluted solution in your laboratory.

10X PBS contains 1.37 M NaCl, 27 mM KCl, 100 mM Na2HPO4, and 18 mM KH2PO4. The final concentration of components in the 1x PBS will be 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4. The pH of 1x PBS will be 7.4.